Technologies
Targeted Proteomics
PBL’s high-throughput targeted mass spectrometry platform, ProteoMarker™, allows for absolute quantification of pre-selected biomarkers with high specificity, selectivity, and accuracy. ProteoMarker™ offers rapid simultaneous measurements of multiple proteins at once (multiplexing), allowing complete independence from antibody-based recognition and availability. Targeted proteomics coupled with our immunoassays can efficiently quantify low-abundant proteins. PBL offers off-the-shelf panels or our experts will help you design and develop new custom assays.
Applications + PBL Expertise
- Protein and peptide absolute quantification
- Biomarker identification
- Biomarker validation
- Multiplex biomarker screening
- Quantification of protein post-translational modifications
- Off-the-shelf and custom assay development
MRM Targeted Proteomics Design
1 - PROTEIN SELECTION
Candidate proteins are selected, based on:
- Project Goals
- Scientific Literature
- DDA/DIA or other discovery data
2 - PEPTIDE SELECTION
How to choose the right peptides:
Resources include online repositories, in-house data, and predictive algorithms.
- Best Flying - Sensitivity
- Unique - Selectivity
Resources include online repositories, in-house data, and predictive algorithms.
3 - TRANSITIONS SELECTION
How to choose the right transitions:
Resources include online repositories and in-house data.
- Most Abundant - Sensitivity
- Unique - Selectivity
Resources include online repositories and in-house data.
Integrated Workflow + Targeted Components
![](https://www.cs-pbl.com/wp-content/uploads/2023/01/targetFLOW2-943x1024.png)